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  4. 3D Transformation Matrix Calculation and Pixel Intensity Normalization for the Dual Focus Tracking System

3D Transformation Matrix Calculation and Pixel Intensity Normalization for the Dual Focus Tracking System

K Sutherland1*, T Hamada2 , M Ishikawa3 , N Miyamoto4 , J Kwon5 , Y Komiya6 , M Mizuta7 , H Date8 , H Shirato9 , (1) ,,,(2) Hokkaido University, Sapporo, Hokkaido, (3) Hokkaido University, Sapporo, Hokkaido, (4) Hokkaido University, Sapporo, Hokkaido, (5) Hokkaido University, Sapporo, Hokkaido, (6) Hokkaido University, Sapporo, Hokkaido, (7) Hokkaido University, Sapporo, Hokkaido, (8) Hokkaido University, Sapporo, Hokkaido, (9) Hokkaido University, Sapporo, Hokkaido

Presentations

(Sunday, 7/29/2018) 3:00 PM - 6:00 PM

Room: Exhibit Hall

Purpose: To provide details of the dual-focal 3D tracking and pixel intensity calibration (DuFT) system used to record gene expression in targeted areas on the skin of freely moving mice.

Methods: Our 3D tracking technique is based on the real-time tumor tracking (RTRT) system used for tracking the motion of tumor surrogate gold markers during radiation treatment.

Results: Our system can automatically record circadian rhythms in clock genes in multiple tissues in unrestrained mice.

Conclusion: Details of the 3D perspective transformation matrix calculation and calibration of pixel value normalization tables are described in this paper.

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