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  5. Evaluation of Biocompatible PEGylated Gold Nanoparticles Versus Citrate-Capped Gold Nanoparticles for Targeting Cervical Cancer in Radiation Therapy

Evaluation of Biocompatible PEGylated Gold Nanoparticles Versus Citrate-Capped Gold Nanoparticles for Targeting Cervical Cancer in Radiation Therapy

S David1*, K Mayer2 , T Gray3 , A Locker4 , D Patel5 , N Kirby6 , (1) University of Texas at San Antonio, San Antonio, TX, (2) University of Texas at San Antonio, San Antonio, TX, (3) The University of Texas at San Antonio, San Antonio, TX, (4) University of Texas at San Antonio, San Antonio, TX, (5) University of Texas at San Antonio, San Antonio, TX, (6) University of Texas HSC SA, San Antonio, TX

Presentations

(Wednesday, 7/17/2019) 10:00 AM - 10:30 AM

Room: Exhibit Hall | Forum 6

Purpose: Compare citrate-capped gold nanoparticles with PEGylated gold nanoparticle to asses their amenability towards synthesis and functionalization in radiotherapy.

Methods: The reduction of tetrachloroauric acid (HAuCl₄) by trisodium citrate (Na₃C₆H₅O₇) to form gold nanospheres was carried out by adding 8 mL of sodium citrate to a boiling gold chloride solution in a glass bottle. The mixture was heated and stirred for an additional 10 minutes until the color of the solution changed to a wine red, which indicates the formation of citrate-capped gold nanoparticles. After the synthesis process, gold nanoparticle (AuNP) samples were characterized by SEM and UV-vis spectroscopy measurements. Thiolated polyethylene glycol (mPEG-SH) of MW 7500 was attached to the surface of the citrate gold nanoparticles via the gold-thiol bond by stirring for 2 hours, allowing citrate ligands to be displaced by the mPEG-SH. The excess PEG was removed by centrifugation at 8000 rpm for 10 minutes followed by decanting the supernatant and resuspending the pellet in DI water. Absorption spectra were obtained on a UV-vis spectrometer.

Results: The UV-vis spectra of the prepared PEGylated AuNPs exhibit an absorption maximum of 536.26 nm. In contrast, the citrate-capped AuNPs resulted in an absorption maximum of 534.17 nm. The redshift of the surface plasmon resonance (SPR) maximum absorption of the spherical AuNPs is a consequence of an increase in the refractive index surrounding the AuNPs due to the modification of AuNPs through PEGylation. SEM imaging of the AuNPs revealed that they are spherical particles approximately 30 nm in dimeter.

Conclusion: Our findings show that we have successfully functionalized spherical gold nanoparticles 30 nm in diameter with the biocompatible polymer PEG, replacing the citrate layer on as-synthesized NP’s. Our future work is to apply these PEG-functionalized nanoparticles to active targeting delivery in in vitro studies of nanoparticle-enhanced radiation therapy for cervical cancer.

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