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Quantification of DNA Damage After An Ionizing Irradiation Session

K Chatzipapas1, P Papadimitroulas2 , N Papanikolaou3 , N Kirby3*, G C Kagadis1 , (1) University of Patras, Rion, Greece, (2) BIOEMTECH,Athens, Greece, (3) University of Texas HSC SA, San Antonio, TX, USA


(Wednesday, 7/17/2019) 10:30 AM - 11:00 AM

Room: Exhibit Hall | Forum 3

Purpose: Ionizing radiation results in DNA lesions that could consequently lead to genetic modifications and/or cell death. This study aims to evaluate some of these lesions by quantifying DNA single strand breaks (SSB), double strand breaks (DSB), as well as clustered DNA damage sites (CDS). Both direct electromagnetic and indirect chemical interactions were investigated. A new parametrization on Geant4-DNA for the quantification was designed.

Methods: Geant4-DNA is used to model DNA molecules and simulate the physical and chemical interactions during ionizing radiation sessions. Moreover, MMB was used to design the pRS-316 DNA molecule (with a length of 4,097 base pairs). This is also used in the prototype DNA dosimeter that was employed in the context of this research, to produce experimental data.New classes have been developed in Geant4-DNA, allowing the implementation of this study’s simulations.The standard model for DNA electromagnetic interactions was used, with no cuts, to make the simulation as detailed as possible.The ARIS HPC infrastructure was used, to speed up the simulations.

Results: The outcome could be summarized as a Geant4 example. This example simulates the irradiation of a DNA molecule, which must be provided in the Protein Data Bank file format. Using ROOT analysis, the number of SSB, DSB, and CDS is calculated depending on the position of energy deposition. The kind of CDS is also available to the end user.

Conclusion: This application provides a tool to extend studies for the biological consequences of ionizing radiation to the scientific community. It can be modified to insert several types of radiation, such as the X-ray spectrum of a LINAC (used in the present study), or the energy spectrum of a radionuclide, etc. Any type of DNA molecule can also be modeled. Capabilities to run a large number of simulations in parallel, such as on clusters, is provided.

Funding Support, Disclosures, and Conflict of Interest: This research is co-financed by Greece and the European Union (European Social Fund- ESF) through the Operational Programme 'Human Resources Development, Education and Lifelong Learning' in the context of the project 'Strengthening Human Resources Research Potential via Doctorate Research' (MiS-5000432), implemented by the State Scholarships Foundation (iky)


Radiobiology, Modeling


TH- Radiobiology(RBio)/Biology(Bio): RBio- general

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