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Evaluation of Nanoparticle Uptake by Cervical and Liver Cancer Cell Lines for Enhanced Radiation Therapy

S David1*, T Gray2, D Patel3, J Valesquez4, N Bassiri7, K Mayer6, N Kirby7, (1) University of Texas at San Antonio, San Antonio, TX, (2) The University of Texas at San Antonio, San Antonio, TX, (3) University of Texas at San Antonio, San Antonio, TX, (4) University Of Texas At San Antonio, ,,(5) ,San Antonio, TX, (6) University of Texas at San Antonio, San Antonio, TX, (7) University of Texas HSC SA, San Antonio, TX

Presentations

(Sunday, 7/12/2020)   [Eastern Time (GMT-4)]

Room: AAPM ePoster Library

Purpose: of biocompatible polymer-coated gold nanoparticles (GNPs) to assess cervical and liver cancer cellular uptake of functionalized GNPs for applications in enhanced radiation therapy


Methods: (C33A) and liver (HEPG2) cancer cells were kept in a 75 mL flask with Eagle’s Minimum Essential Medium (EMEM), 10% Fetal Bovine Serum (FBS), and Penicillin and were incubated at 37°C and 5% CO2. After incubation, cells were plated with PEGylated GNPs and allowed to incubate for 24 hours. Afterwards, cells were fixed, and confocal fluorescence imaging was carried out to examine GNP uptake with an excitation wavelength of 514 nm using a tunable Argon-ion laser. Images were assessed to compare the condition of cell/nanoparticle interactions between cancerous and normal cervical and liver cell lines for use in GNP enhanced radiation therapy.


Results: UV-vis spectra of the prepared PEGylated AuNPs exhibit an absorption maximum of 536.26 nm. In contrast, the citrate-capped AuNPs resulted in an absorption maximum of 534.17 nm. SEM imaging of the GNPs revealed that they are spherical particles approximately 30 nm in diameter. Confocal images show uptake of the PEGylated GNPs 24 hours after incubation. The data was obtained with ImageJ analysis, which indicates increased uptake of the GNPs.


Conclusion: findings show that we have successfully functionalized the gold nanoparticles 30 nm in diameter with the biocompatible polymer PEG. A protocol for bioconjugation of PEGylated GNPs with anti-EGFR antibodies has been presented. Confocal images show that 30 nm GNPs were distributed inside the cell cytoplasm, surrounding the nucleus. Our future work is to apply these PEG-functionalized nanoparticles to active targeting delivery in in vitro studies of nanoparticle-enhanced radiation therapy for cervical cancer.

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Funding Support, Disclosures, and Conflict of Interest: NIH-funded RISE grant

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