(Sunday, 7/12/2020) [Eastern Time (GMT-4)]
Room: AAPM ePoster Library
Purpose:
The present concept of radiobiology posits that damage to DNA in the cell nucleus is the primary cause for the determinate effects of radiation. However, emerging experimental evidence suggests that this thermotical framework is insufficient for describing extranuclear radiation effect, mainly the response to Mitochondria. To investigate whether irradiation-induced mitochondrial dysfunction contributes to the increase in the production of ROS in mitochondria of Human prostate cell line with and without gold nanoparticles, we measured the levels of ROS in cells with the MitoSOX Red mitochondrial superoxide indicator as a specific probe of mitochondrial O_2^-.
Methods:
We used DU-145 for this study. Cells were divided in two group, the group that there is cell only and the group that gold nanoparticle has been injected 24 hours before radiation. The cells were cultured in EMEM supplemented with 10% FBS at 37 ° C under a humidified atmosphere of 5% CO2. The cells at approximately 80% confluence. Cells were irradiated with 6 MV x-ray energies from a Linac machine at various radiation doses (0, 2, 4, 6, Gy). 24, 48 and 72 hours after radiation the level of ROS in the mitochondria was analysed by flow cytometry with a MitoSOX Red mitochondrial superoxide indicator.
Results:
After the exposure of cells to gamma-ray irradiations, we found that the irradiation induced the following changes in a clear pattern of time courses. a significant increase of ROS levels in the mitochondria were observed in cells treated with GNP and exposed to 4Gy irradiation 48 hours post irradiation.
Conclusion:
Results in this study prove that the combination of gold nanoparticle and ionizing radiation increases the level ROS in mitochondria and eventually enhances the damage on mitochondria of cancer cells.
Keywords
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Not Applicable / None Entered.
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