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Biochemical Evaluation of Radiotherapy-Induced Small Intestinal Damage: Melatonin as Radioprotector

D Shabeeb1 , A Musa2*, G Omyan3 , (1) Department of Physiology, College of Medicine, University of Misan, Iraq. (2) Department of Medical Physics, Tehran University of Medical Sciences, International Campus, Tehran, Iran. (3) Radiotherapy Oncology Research Center, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran.

Presentations

(Sunday, 7/14/2019)  

Room: ePoster Forums

Purpose: To evaluate, using biochemical analysis, the radioprotective effect of melatonin against radiotherapy-induced small intestinal damage.

Methods: In this study, 20 male Wistar rats were randomly divided to four groups (5 rats in each); group 1 (control, without radiation or melatonin treatment), group 2 (melatonin treated), group 3 (radiation only) and group 4 (melatonin + radiation). The abdominal regions of animals in groups 3 and 4 were exposed to 8 Gy single radiation dose from a cobalt-60 gamma ray source. Thirty minutes before irradiation, groups 2 and 4 rats were administered 100 mg/kg of melatonin via intraperitoneal route. All rats were sacrificed 5 days after irradiation. Afterwards, their small intestinal tissues were carefully removed and kept for biochemical analysis using the following parameters: superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA).

Results: Biochemical evaluations of rats’ intestinal tissues indicated significantly higher MDA levels in the radiation group compared to the radiation + melatonin group (p < 0.0006). In addition, both CAT and SOD levels were significantly decreased in the radiation group compared to the radiation + melatonin group (p < 0.05). In all biochemical assessments, we observed no significant difference between the control group and the radiation + melatonin group (p > 0.05). Furthermore, there were no side effects from melatonin administration.

Conclusion: Melatonin administration prior to irradiation reduced MDA levels as well as increased both CAT and SOD levels. Therefore, melatonin can protect against radiotherapy-induced small intestinal damage.

Keywords

Radiation Therapy, Radiation Protection, Ionizing Radiation

Taxonomy

TH- Radiobiology(RBio)/Biology(Bio): RBio- general

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