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Raman Spectroscopy for Assessing Normal and Cancer Response in Vitro Following Radiation Exposure

S Devpura1*, S Brown1 , Y Zhang1 , K Barton1 , F Siddiqui1 , S Sethi2 , G Divine1 , A Pandya3 , I Chetty1 , (1) Henry Ford Health System, Detroit, MI, (2) University of Michigan VA, Ann Arbor, MI, (3) Wayne State University, Detroit, MI


(Wednesday, 8/1/2018) 7:30 AM - 9:30 AM

Room: Karl Dean Ballroom B1

Purpose: To characterize the radiation-induced biochemical responses in normal and cancer cells spectroscopically using established lung and prostate cells.

Methods: Nine cells lines were investigated, including 4 human lung cells lines including fibroblasts (CCL186) and epithelial, squamous or adeno-squamous cancer cells (A549, CRL5928, and HTB178), 4 human prostate cells lines (two normal prostate epithelial (PCS440010 and CRL11610) and two prostate cancer (CRL2505 and DU145)) and 1 murine cell line (TRAMPC2). Cells were irradiated with 2, 5, 10, 15 and 30 Gy single fraction using a Cs-137 irradiator. A total of 1492 Raman spectra were acquired as a function of time post-radiation (24, 48 or 72 hrs). Principal component analysis (PCA) and discriminant function analysis (DFA) were used to analyze the Raman spectral features.

Results: PCA captured 95-97% of the variance in the Raman spectral features for all the cell lines. Overall, PCA/DFA analysis showed unirradiated cells were correctly classified in the range of 72-100% depending on the type of cells and the time point. All irradiated lung and prostate cells (normal or cancer) were classified with 79.0±13.6% accuracy. Unirradiated prostate normal cells were correctly identified in the range of 95-100% and lung fibroblast cells were correctly identified in the range of 84-100%. Unirradiated human prostate carcinoma cells and murine prostate cells were classified with an accuracy of > 88% and 93%, respectively. Unirradiated lung cancer cells were classified in the range of 72-100%. Multiple Raman bands assignable to DNA/RNA, protein and lipids showed prominent contributions in cell differentiation between unirradiated and irradiated with different doses.

Conclusion: 2 Gy is sufficient to characterize radiation-induced Raman features per PCA/DFA analysis for the majority of cell lines studied with a high degree of accuracy. Accuracy improved at the later time points, 48 and 72 hr relative to 24 hr in 4 cell lines.

Funding Support, Disclosures, and Conflict of Interest: This work is supported by JFCI CRAG internal pilot funding, Henry Ford Health System.


Spectrometry, Lung, Prostate Therapy


IM- Optical : Spectroscopy

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